Reuse masks and saved meshes ============================ Resume at different points in the workflow, provided the inputs and saved geometry belong to the same fields and calibration. Reuse existing masks -------------------- .. code-block:: python from bactoscoop import ImageCollection ic = ImageCollection(r"C:\path\to\data", px=0.065) ic.create_image_objects(phase_channel="C1") ic.batch_process_mesh(phase_channel="C1", save_data=True) ``create_image_objects`` loads missing phase images and masks, validates their field identities, and creates new image objects. In the tutorials, set ``RUN_SEGMENTATION=False`` to take this route. Reuse geometry -------------- .. code-block:: python ic = ImageCollection(r"C:\path\to\data", px=0.065) ic.batch_load_mesh("data_curated_meshdata.pkl", phase_channel="C1") # Continue with channel detection and feature extraction. ``batch_load_mesh`` accepts pickle and reloadable mesh Parquet. ``pkl_path`` can specify the directory containing the mesh file; its default is the image folder. The image/mask files are still needed to reconstruct image objects and measure signals. The file needs compatible geometry columns and field identities. State after loading ------------------- Reloading creates a new image/cell population and clears previous feature and merged tables. Saved mesh tables preserve geometry, not all channel caches, detected objects, or feature dictionaries. Redetect objects and calculate the requested features after reloading. Verify ``px`` against the original experiment rather than assuming the saved geometry file carries all acquisition settings. See :py:meth:`bactoscoop.imagecollection.ImageCollection.batch_load_mesh`.